Strategies to enhance the ability of nerve guidance conduits to promote directional nerve growth

Table 1 Methods to test the ability of NGCs to promote nerve regeneration

Experiment types	Methods	References
Cell culture in vitro	Cell morphology observation	[21, 22, 23, 24, 25, 26]
	Detection of differentiation-related substances	[27, 28, 29, 30]
	Detection of myelination-related substances	[31]
Repair of rat sciatic nerve defects in vivo	Histologic section observation	[12, 32, 33]
	Immunofluorescence staining	[29, 34]
	T2 signal changes in MRI of injured nerves	[35]
	Electrophysiological signal detection	[12, 33, 34]
	Reflex function measurement	[12, 33]
	Motor function measurement	[12, 33, 34]
	Atrophic gastrocnemius muscle weighing	[12, 33]

ISSN: 1475-925X