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Table 1 Primer sequences of the genes analysed by RT-PCR

From: Effects of dynamic radial tensile stress on fibrocartilage differentiation of bone marrow mesenchymal stem cells

Gene names

Forward (5′–3′)

Reverse (5′–3′)

Col I

CCTGCGTACAGAACGGCCT

ACAGCACGTTGCCGTTGTC

Col II

AGCAGCAAGAGCAAGGACAAG

TCTTGCAGTGGTAGGTGATGTT

α-SMA

ATAACCCTCCAGCCTTCAGC

TCCCATTCCCACCATCACT

GAGs

GTCCACCATTCGGCATAACC

TGGGGTCACTTCAACCAAACT

GAPDH

CAAGTTCCACGGCACAGTCA

GGTTCACGCCCATCACAAA

  1. The genes of Col I, Col II, GAGs and α-SMA were analysed by RT-PCR. GAPDH was the internal reference gene used to normalize the relative expression level of other protein genes
  2. Col I, type I collagen; Col II, type II collagen; GAGs, glycosaminoglycans; α-SMA, α-smooth muscle actin