Fig. 5From: Image-based quantitative analysis of tear film lipid layer thickness for meibomian gland evaluationSpatial visualization of lipid layer thickness distribution. a–c The ROI region for each patient at a selected frame d–f 3D plots displaying each patient’s lipid layer thickness in a 50 × 50-pixel grid (i.e. 2500 pixels). The entire ROI contains approximately 5000–7000 pixels. Patient I has hyposecretory MGD (a, d), patient II has dry eye without MGD (b, e), and patient III has hypersecretory MGD (c, f)Back to article page