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Table 1 Experimental details for the five different decellularization protocols

From: Preparation and characterization of small-diameter decellularized scaffolds for vascular tissue engineering in an animal model

Protocol step

Group

I

II

III

IV

V

Soaking in ddw for 24 h

√

√

√

√

√

Frozen at −80 °C and thawed twice

√

√

√

√

√

Soaking: 75% ethanol

Solvent:tissue ratio: 20:1 (v/w)

Total duration: 72 h

Solvent replacement at 1, 3, 6, 12, 24, 72 h

√

√

√

√

√

Soaking in ddw for 24 h

√

√

√

√

√

Digestion on an orbital shaker using a tissue:enzyme ratio (g/ml) of 1:15

0.125% pepsin

0.125% pepsin

0.125% pepsin

0.125% pepsin

0.125% pepsin

Solvent

1× EDTA

2× EDTA

1× EDTA

1× EDTA

2× EDTA

Duration (h)

1.5

1

2

2

1.5

PBS washing for 30 min; shaking

√

√

√

√

√

DNase and RNase for 6 h; shaking

√

√

√

√

√

Rinsing solution

ddw

ddw

Triton

ddw

Triton

Duration (h)

1

1

2

1

2

Ultraviolet irradiation for 30 min

   

√

√

  1. DNase and RNase concentration was 70 U/ml, respectively
  2. Each step was performed at 4 °C
  3. n = 5 for each group, repeated six times in all
  4. ddw double-distilled water, Triton 1% Triton X100