Figure 6

Polarized light images of collagen gel response to in vitro acupuncture after 2 needle revolutions (A-C), and 4 revolutions (D-F) in untreated collagen gels and 2 revolutions (G-I) in crosslinked gels. The birefringent area increased with increasing revolutions, and was greater in untreated collagen compared to crosslinked collagen at the same number of revolutions. This area also increased with increasing collagen concentration for each condition (A, D, G – 1.5 mg/ml; B, E, H – 2.0 mg/ml; C, F, I – 2.5 mg/ml). Bar = 1 mm.