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Table 1 Flow rates needed for 1 dyn/cm2 shear stress, magnification used and camera shutter speed used for each of the three chambers tested (including the Oligene chamber with the cells seeded on the coverslip). Note: The eyepiece objective of the microscope used was 1×.

From: The imperative for controlled mechanical stresses in unraveling cellular mechanisms of mechanotransduction

Chamber

Flow rate (ml/min) for 1 dyn/cm2

Magnification

Camera Shutter Speed (ms)

Oligene FCS

0.774

20×

20

Oligene FCS (with cells)

0.774

20×

20

Bioptechs FCS2

3.624

10×

20

Warner RC-30 HV

1.278

10×

10