Figure 4
From: Automatic tracking of cells for video microscopy in patch clamp experiments
Alpha blending and neuron tracking. (A) Image obtained from Dodt Contrast Microscopy. The arrow indicates a neuron of interest. (B) Image of the cellular response to fluorescent light on the same region of (A). (C) Real time overlay using Alpha Blending technique between (A) and (B). The white square in (D), (E) and (F) represents the neuron tracking applied to three displaced images. Blending between fluorescence information and ROI is dinamically performed and highlighted in red.