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Figure 1 | BioMedical Engineering OnLine

Figure 1

From: Automatic tracking of cells for video microscopy in patch clamp experiments

Figure 1

UML activity diagram. The first step of the diagram is the acquisition of frames (30 fps) from the camera that is coupled to the microscope. Light on means that the fluorescent light is turned on, and therefore the image displays information of cell fluorescence. Light off means that the fluorescent light is turned off. Select ROI (Region of Interest) permits the user to select a ROI in the image, to be used in neuron tracking and overlay steps. Acquire New Image performs the acquisition of the next frame. Tracking step performs ROI tracking in subsequent images. Overlay means to accomplish blending between the current image and the fluorescence information (acquired in light on step) of ROI during tracking.

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