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Figure 3 | BioMedical Engineering OnLine

Figure 3

From: Ratiometric imaging of calcium during ischemia-reperfusion injury in isolated mouse hearts using Fura-2

Figure 3

Ligation of the left anterior descending artery produces a pronounced myocardial infarction. Following cannulation of the aorta, a suture was inserted under the LAD and gently looped without applying pressure to the surface of the heart. Hearts were then loaded with Fura-2 AM and continuously paced at a cycle length of 150 ms near the base; Ca2+ transients were recorded from the surface of the left ventricle. (A, left) Initially, the diastolic Ca2+ is unchanged across the heart and calcium transients above and below the suture are identical. (A, middle) When the suture is tied off to create an MI, the diastolic Ca2+ rises dramatically in the ischemic region while staying normal in the region above the suture. (A, right) When the suture is untied and the MI is reperfused, diastolic calcium returns to normal as do the Ca2+ transients. (B and C) Transients from the well-perfused region above the suture stay consistent throughout the experiment, whereas ligation of the LAD caused significant reversible changes to diastolic Ca2+ and transient shape.

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